SCILLS - The Scottish Institute for Cell Signalling

Goh, E.T.H., Arthur, J.F.C.,  Cheung, P.C.F., Akira, S., Toth, R. and Cohen, P. (2012). Identification of the protein kinases that activate the E3 ubiquitin ligase Pellino 1 in the innate immune system. Biochem J 441, 339-346.

Alexandru, G., Pariente, N., and Xirodimas, D. (2011). In the family with ubiquitin.
EMBO Rep 12, 880-882.

Hjerpe, R., Thomas, Y., Chen, J. Zemla, A., Curran, S., Shpiro, N., Dick, R.L., and Kurz, T. (2011).  Changes in the ratio of free Nedd8 to Ubiquitin triggers neddylation by ubiquitin enzymes.  Biochem J 441, 927-936.

Rabut, G., Le Dez, G., Verma, R., Makhnevych, T., Knebel, A., Kurz, T., Boone, C., Deshaies, R.J. and Peter, M. (2011).  The TFIIH subunit Tfb3 regulates cullin neddylation. Mol Cell 5, 488-495.

Larance, M., Bailly, A.P., Pourkarimi, E., Hay, R.T., Buchanan, G., Coulthurst, S., Xirodimas, D.P., Gartner, A., and Lamond, A.I. (2011). Stable-isotope labeling with amino acids in nematodes. Nat Methods 8, 849-851.

Matic, I., Jaffray, E.G., Oxenham, S.K., Groves, M.J., Barratt, C.L., Tauro, S., Stanley-Wall, N.R., and Hay, R.T. (2011). Absolute SILAC-Compatible Expression Strain Allows Sumo-2 Copy Number Determination in Clinical Samples. J Proteome Res 10, 4869-4875.

Plechanovova, A., Jaffray, E.G., McMahon, S.A., Johnson, K.A., Navratilova, I., Naismith, J.H., and Hay, R.T. (2011). Mechanism of ubiquitylation by dimeric RING ligase RNF4. Nat Struct Mol Biol 18, 1052-1059.

Tatham, M.H., Matic, I., Mann, M., and Hay, R.T. (2011). Comparative proteomic analysis identifies a role for SUMO in protein quality control. Sci Signal 4, rs4.

Clark, K., Takeuchi, O., Akira, S. and Cohen, P. (2011). TANK facilitates crosstalk within the IKK family during TLR signaling. Proc Natl Acad Sci (USA) 108, 17093-17098.

Gleason, C.E., Ordureau, A., Gourlay, R., Arthur, J.S.C. and Cohen, P. (2011). Polyubiquitin binding to optineurin is required for optimal activation of TANK-binding kinase 1 and the production of interferon β. J Biol Chem 286, 35663-35674

Nanda, S.K., Venigalla, R.K., Ordureau, A., Patterson-Kane, J.C., Powell, D.W., Toth, R., Arthur, J.S.C., and Cohen, P. (2011). Polyubiquitin binding to ABIN1 is required to prevent autoimmunity. J Exp Med 208, 1215-1228.

Clark, K., Peggie, M., Plater, L., Sorcek, R.J., Young, E.R., Madwed, J.B., Hough, J., McIver, E.G., and Cohen, P. (2011). Novel cross-talk within the IKK family controls innate immunity. Biochem J 434, 93-104.

Bruderer, R., Tatham, M.H., Plechanovova, A., Matic, I., Garg, A.K., and Hay, R.T. (2011). Purification and identification of endogenous polySUMO conjugates. EMBO Rep 12, 142-148.

Napolitano, L.M., Jaffray, E.G., Hay, R.T., and Meroni, G. (2011). Functional interactions between ubiquitin E2 enzymes and TRIM proteins. Biochem J 434, 309-319.

Smith, H., Liu, X.Y., Dai, L., Goh, E.T., Chan, A.T., Xi, J., Seh, C.C., Qureshi, I.A., Lescar, J., Ruedl, C., Gourlay, R., Morton, S., Hough, J., McIver, E.G., Cohen, P. and Cheung, P.C.F. (2011). The role of TBK1 and IKKε in the expression and activation of Pellino 1. Biochem J 434, 537-548.

Hattersley, N., Shen, L., Jaffray, E.G., and Hay, R.T. (2011). The SUMO protease SENP6 is a direct regulator of PML nuclear bodies. Mol Biol Cell 22, 78-90.

Cohen, P., and Tcherpakov, M. (2010). Will the ubiquitin system furnish as many drug targets as protein kinases? Cell 143, 686-693.

Piwko, W., Olma, M.H., Held, M., Bianco, J.N., Pedrioli, P.G., Hofmann, K., Pasero, P., Gerlich, D.W., and Peter, M. (2010). RNAi-based screening identifies the Mms22L-Nfkbil2 complex as a novel regulator of DNA replication in human cells. EMBO J 29, 4210-4222.

Li, X., Vadrevu, S., Dunlop, A., Day, J., Advant, N., Troeger, J., Klussmann, E., Jaffrey, E., Hay, R.T., Adams, D.R., Houslay, M.D. and Baillie, G.S. (2010). Selective SUMO modification of cAMP-specific phosphodiesterase-4D5 (PDE4D5) regulates the functional consequences of phosphorylation by PKA and ERK. Biochem J 428, 55-65.

Hjerpe, R., Aillet, F., Lopitz-Otsoa, F., Lang, V., Torres-Ramos, M., Farras, R., Hay, R.T., and Rodriguez, M.S. (2010). Oligomerization conditions Mdm2-mediated efficient p53 polyubiquitylation but not its proteasomal degradation. Int J Biochem Cell Biol 42, 725-735.

Golebiowski, F., Tatham, M.H., Nakamura, A., and Hay, R.T. (2010). High-stringency tandem affinity purification of proteins conjugated to ubiquitin-like moieties. Nat Protoc 5, 873-882.

Hannoun, Z., Greenhough, S., Jaffray, E., Hay, R.T., and Hay, D.C. (2010). Post-translational modification by SUMO. Toxicology 278, 288-293.

Castillo-Lluva, S., Tatham, M.H., Jones, R.C., Jaffray, E.G., Edmondson, R.D., Hay, R.T., and Malliri, A. (2010). SUMOylation of the GTPase Rac1 is required for optimal cell migration. Nat Cell Biol 12, 1078-1085.

Geoffroy, M.C., Jaffray, E.G., Walker, K.J., and Hay, R.T. (2010). Arsenic-induced SUMO-dependent recruitment of RNF4 into PML nuclear bodies. Mol Biol Cell 21, 4227-4239.

Scott, D.C., Monda, J.K., Grace, C.R., Duda, D.M., Kriwacki, R.W., Kurz, T., and Schulman, B.A. (2010). A dual E3 mechanism for Rub1 ligation to Cdc53. Mol Cell 39, 784-796.

Bailly, A.P., Freeman, A., Hall, J., Declais, A.C., Alpi, A., Lilley, D.M., Ahmed, S., and Gartner, A. (2010). The Caenorhabditis elegans homolog of Gen1/Yen1 resolvases links DNA damage signaling to DNA double-strand break repair. PLoS Genet 6, e1001025.

Siergiejuk, E., Scott, D.C., Schulman, B.A., Hofmann, K., Kurz, T., and Peter, M. (2009). Cullin neddylation and substrate-adaptors counteract SCF inhibition by the CAND1-like protein Lag2 in Saccharomyces cerevisiae. EMBO J 28, 3845-3856.

Cohen, P. (2009). Keep nibbling at the edges. J Biol Chem 284, 23891-23901.

Smith, H., Peggie, M., Campbell, D.G., Vandermoere, F., Carrick, E., and Cohen, P. (2009). Identification of the phosphorylation sites on the E3 ubiquitin ligase Pellino that are critical for activation by IRAK1 and IRAK4. Proc Natl Acad Sci USA 106, 4584-4590.

Clark, K., Plater, L., Peggie, M., and Cohen, P. (2009). Use of the pharmacological inhibitor BX795 to study the regulation and physiological roles of TBK1 and IκB kinase ε: a distinct upstream kinase mediates Ser-172 phosphorylation and activation. J Biol Chem 284, 14136-14146.

Cohen, P. (2009). Targeting protein kinases for the development of anti-inflammatory drugs. Curr Opin Cell Biol 21, 317-324.

Geoffroy, M.C., and Hay, R.T. (2009). An additional role for SUMO in ubiquitin-mediated proteolysis. Nat Rev Mol Cell Biol 10, 564-568.

Golebiowski, F., Matic, I., Tatham, M.H., Cole, C., Yin, Y., Nakamura, A., Cox, J., Barton, G.J., Mann, M., and Hay, R.T. (2009). System-wide changes to SUMO modifications in response to heat shock. Sci Signal 2, ra24.

Shen, L.N., Geoffroy, M.C., Jaffray, E.G., and Hay, R.T. (2009). Characterization of SENP7, a SUMO-2/3-specific isopeptidase. Biochem J 421, 223-230.

Tatham, M.H., and Hay, R.T. (2009). FRET-based in vitro assays for the analysis of SUMO protease activities. Methods Mol Biol 497, 253-268.

Meyer-Schaller, N., Chou, Y.C., Sumara, I., Martin, D.D., Kurz, T., Katheder, N., Hofmann, K., Berthiaume, L.G., Sicheri, F., and Peter, M. (2009). The human Dcn1-like protein DCNL3 promotes Cul3 neddylation at membranes. Proc Natl Acad Sci USA 106, 12365-12370.

Rosado, I.V., Niedzwiedz, W., Alpi, A.F., and Patel, K.J. (2009). The Walker B motif in avian FANCM is required to limit sister chromatid exchanges but is dispensable for DNA crosslink repair. Nucleic Acids Res 37, 4360-4370.

Moser, S.C., von Elsner, S., Bussing, I., Alpi, A., Schnabel, R., and Gartner, A. (2009). Functional dissection of Caenorhabditis elegans CLK-2/TEL2 cell cycle defects during embryogenesis and germline development. PLoS Genet 5, e1000451.

Alpi, A.F., and Patel, K.J. (2009). Monoubiquitylation in the Fanconi anemia DNA damage response pathway. DNA Repair (Amst) 8, 430-435.